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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC
Phosphoinositide-specific phospholipase C (PLC) is a family of proteins that hydrolyze phosphatidylinositol 4,5-bisphosphate to generate the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). IP3 induces a transient increase in intracellular free Ca++, while DAG is a direct activator of protein kinase C (PKC). These processes are involved in the control of multiple cellular activities such as cell proliferation, growth and differentiation. PLCy-1 is a 135kD ubiquitously expressed isozyme that is activated by phosphorylation of tyrosine 783 and interacts with growth factor receptors (EGFR, PDGFR, etc.) and adhesion/cytoskeletal proteins (FAK, paxillin) to influence a multiple number of signaling pathways.
Catalog #P4074-42T
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 dilution.
Optimal dilutions to be determined by the researcher.
Positive ControlNIH3T3 cells +/- PDGF.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phosphopeptide derived from the region of human PLCy-1 that contains tyrosine 783.
SpecificityRecognizes human PLCy-1 when phosphorylated at Tyr783. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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