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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

Anti -Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3). Phosphorylation of PLC-gamma1 Y783 by EGF causes the conformational change of PLC-gamma1 to allow the interaction of its SH3 domain with Akt proline-rich motifs. Interaction between PLC-gamma1 and Akt enhanced EGF-stimulated cell motility (4).
Catalog #P4074-42V1
ApplicationsSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:100,000
Immunocytochemistry: 1:250-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E437
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA phospho-specific peptide corresponding to residues surrounding Tyrosine 783.
SpecificityRecognizes human PLC gamma 1 phosphorylated on tyrosine 783.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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