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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C -1 (PLC 1) (Tyr783), phosphorylated

Anti -Phospholipase C -1 (PLC 1) (Tyr783), phosphorylated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P4074-42Z
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quality Control Testing Immunoblot Analysis A 1:1,000 dilution of this lot detected phospho-PLC 1 (Tyr783) in lysate from PDGF treated NIH3T3 cells. Immunoblot Analysis Lysates from untreated (lane 1) and PDGF treated (lanes 2 and 3) NIH3T3 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with antiphospho- PLC 1 (Tyr783). Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-PLC 1 (Tyr783). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho- PLC 1.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.2, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, rat, cow and frog phospho-Phospholipase C -1 (Tyr783).
SpecificityRecognizes phospho-Phospholipase C -1 (Tyr783), Mr. 135. Species Cross-reactivity: Human and Mouse tested. Predicted to cross-react with other species (100% homologous) based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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