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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 1 Phosphorylated, (Tyr1245)

Anti -Phospholipase C, gamma 1 Phosphorylated, (Tyr1245)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IC
The phospholipase C gamma-1 (PLC gamma I) is a member of phospholipase C family which catalyzes the hydrolysis of phospholipids to yield second messengers such as diacylglycerols (DAG) and water-soluble phosphorylated derivatives of the lipid head groups. These second messengers play a crucial role in transmembrane signaling and are involved in the regulation of several cellular functions, such as cell differentiation and proliferation (1). PLC gamma I interacts with variety of signaling molecules via its two SH2 domains and a single SH3 domain and it is activated by phosphorylation on Tyr783 through interaction with non-receptor and receptor tyrosine kinases. Interaction with activated receptors (e.g. PDGFR and EGFR) leads to phosphorylation on Tyr771, Tyr783, and Tyr1245 (2). Phosphorylations at Tyr783 and 1245 might be significant for adhesion PLC gamma I to fibronectin during integrin initiated fibronectin adhesion (3)
Catalog #P4074-43B
ApplicationsSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:2,000-1:5,000
Immunocytochemistry: 1: 100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E439
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residue surrounding tyrosine 1245 of human PLC gamma I.
SpecificityRecognizes human PLC gamma I, phosphorylated (pY1245).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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