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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 2, phosphorylated (Y1197) (PLCg2)

Anti -Phospholipase C, gamma 2, phosphorylated (Y1197) (PLCg2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant E B IP IC
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate (1). PLCgII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgII (2). Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk (3).
Catalog #P4074-45E
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:2000-1:20,000
Immunocytochemistry: 1:100-1:250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No8k7
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
PuritySupernatant
ImmunogenA phospho specific peptide corresponding to residues surrounding tyrosine 1197 of human PLCg2.
SpecificityRecognizes human PLCg2 when phosphorylated on tyrosine 1197.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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