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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase C, gamma 2, phosphorylated (Tyr1217)

Anti -Phospholipase C, gamma 2, phosphorylated (Tyr1217)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups. The phospholipase C gamma-2 (PLCgII) is an enzyme that plays a crucial role in intracellular signal transduction pathways. This enzyme is important because of its role in the generation of second messengers following the hydrolysis of phosphatidylinositol 4,5-bisphosphate. PLCgII has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. It has been suggested that tyrosine kinases activate PLCgII. Btk-deficient B cells exhibited a marked reduction in phosphorylation of the Src homology 2 (SH2)-SH3 linker region sites, Tyr(753) and Tyr(759). Phosphorylation of both sites was restored by expression of Tec, but not Syk, family kinases. In contrast, phosphorylation of the PLCgamma2 carboxyl-terminal sites, Tyr(1197) and Tyr(1217), was unaffected by the absence of functional Btk.
Catalog #P4074-45H
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Paraffin sections): 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthesized phosphopeptide derived from human PLC-gamma2 around the phosphorylation site of tyrosine 1217 (F-L-pY-D-T).
SpecificityRecognizes endogenous levels of human PLC-gamma 2 only when phosphorylated at tyrosine 1217.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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