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You are here:Home » Antibodies » Antibodies-Enzymes, Phospholipase » Anti -Phospholipase D1, phosphorylated (PLD1) (Ser561)

Anti -Phospholipase D1, phosphorylated (PLD1) (Ser561)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified. Both are regulated by protein kinases, small GTPases and Ca2+ (1). PLD1 is phosphorylated at serine 2, serine 561 and threonine 147 by PKC (2,3). Phosphorylation at threonine 147 and serine 561 regulates PLD1 activity (3).
Catalog #P4074-50A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot deremined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser561 of human PLD1.
SpecificityDetects endogenous levels of human PLD1 only when phosphorylated at serine 561. Does not crossreact with phosphorylated PLD2. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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