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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -Phospholipase D1, phosphorylated (Thr147) (PLD1)

Anti -Phospholipase D1, phosphorylated (Thr147) (PLD1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA). PA is the precursor of the second messenger, diacylglycerol (DAG). Two isoforms of PLD (PLD1 and PLD2) have been identified. Both are regulated by protein kinases, small GTPases and Ca2+. PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC. Phosphorylation at Ser147 and Thr561 activates PLD1 activity.
Catalog #P4074-50B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000. Band is observed at ~120kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1.
SpecificityDetects endogenous levels of human PLD1 phosphorylated at Thr147. Does not crossreact with phosphorylated PLD2.
Species CrossreactivityMouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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