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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PHPT1, Recombinant, Human (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase 1, Protein Janus-A Homolog, PHP14, CGI-202, HSPC141

PHPT1, Recombinant, Human (14kD Phosphohistidine Phosphatase, Phosphohistidine Phosphatase
1, Protein Janus-A Homolog, PHP14, CGI-202, HSPC141

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Specifications

PHPT1 is a 125aa enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
Catalog #P4074-54M
SourceRecombinant corresponding to aa1-125 of human PHPT1 protein, fused to His-tag at N-terminus, expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Molecular Weight 15.9kD (145aa) confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight15.9kD
SourceE.coli
Purity~95% (SDS-PAGE).
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 2mM DTT, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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