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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #P4075-27
ApplicationsCan be used for immunoassay and Western Blot.
Recommended DilutionWestern Blot: 1:10. Western Blot can be performed using standard procedures. Blocking should be performed with 1-1.5% bovine serum albumin (for phosphotyrosine blotting never use non-fat dry milk or other).
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A612
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
PurityPurified IgG
SpecificityReacts with phosphotyrosine. It does not crossreact with phosphoserine or phosphothreonine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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