Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

Specific reversible protein phosphorylation plays a central role in regulating many cellular events by controlling the activity of participating proteins. Ways that phosphorylation/dephosphorylation can modulate a protein’s activity include altering its conformation, localization, stability, and ability to associate or form complexes with other proteins(3-5). As much as one third of cellular proteins may be phosphorylated at any given moment. Reversible protein tyrosine phosphorylations are central to regulating events in signal transduction pathways initiated by most growth factors and cytokines.
Catalog #	P4075-28
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 0.5-1ug/ml
	Immunoprecipitation: 2-5ug
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	LgG2b
Clone No	3G239
Host	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in 10mM sodium phosphate, pH 7.4, and 0.05% sodium azide, before the addition of glycerol to 40%.
Purity	Purified by Protein A affinity chromatography.
Immunogen	KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine.
Specificity	Recognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.