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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Specific reversible protein phosphorylation plays a central role in regulating many cellular events by controlling the activity of participating proteins. Ways that phosphorylation/dephosphorylation can modulate a proteinís activity include altering its conformation, localization, stability, and ability to associate or form complexes with other proteins(3-5). As much as one third of cellular proteins may be phosphorylated at any given moment. Reversible protein tyrosine phosphorylations are central to regulating events in signal transduction pathways initiated by most growth factors and cytokines.
Catalog #P4075-28
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml
Immunoprecipitation: 2-5ug
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeLgG2b
Clone No3G239
HostMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in 10mM sodium phosphate, pH 7.4, and 0.05% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH coupled with equal amounts of phosphotyrosine, glycine, and alanine.
SpecificityRecognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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