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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine (HRP)

Anti -Phosphotyrosine (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation.
Catalog #P4075-31
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:2000
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 1% BSA 0.1% Proclin, 40% glycerol. Labeled with Horseradish peroxidase (HRP) (RZ > 3.0).
PurityPurified by immunoaffinity chromatography
ImmunogenPurified tyrosine-phosphorylated proteins.
SpecificityRecognizes phosphotyrosine-containing proteins. It has minimal or no reactivity with phosphothreonine or phosphoserine-containing proteins. It will react with tyrosine-phosphorylated proteins from any species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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