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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation.
Catalog #	P4075-32
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Can also be used for conjugation to label including enzymes, fluorochromes, biotin and agarose. Other applications not tested.
Recommended Dilutions	ELISA: 1:5000-10,000
	Western Blot: 1:1000-1:2000
	Immunoprecipitation: 2-5ug/IP reaction
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Affinity purified native tyrosine phosphorylated proteins
Specificity	Recognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
Species Crossreactivity	Tyrosine-phosphorylated proteins from any species
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.