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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation.
Catalog #P4075-32
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Can also be used for conjugation to label including enzymes, fluorochromes, biotin and agarose. Other applications not tested.
Recommended DilutionsELISA: 1:5000-10,000
Western Blot: 1:1000-1:2000
Immunoprecipitation: 2-5ug/IP reaction
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenAffinity purified native tyrosine phosphorylated proteins
SpecificityRecognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins.
Species CrossreactivityTyrosine-phosphorylated proteins from any species
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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