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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the posttranslational state of a broad range of phosphotyrosine containing proteins. Immunoprecipitation of proteins of interest followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Catalog #	P4075-33
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation or for antigen applications in immunological protocols. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
Specificity	Detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.