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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.(3,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes, including survival, proliferation, differentiation, and transformation.(3,6,7) The use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization, and purification of proteins that are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).(1,2,4,5)
Catalog #	P4075-35
Applications	Suitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blotting: 1:500 to 1:1,000
	Immunoprecipitation:
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G244
Host	Mouse
Concentration	As reported
Form	Supplied as a liquid in phosphate buffered saline, pH 7.4, 40% glycerol and 0.1% proclin.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Tyrosine-phosphorylated proteins
Specificity	PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective.
Species crossreactivity	It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosinephosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.