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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine, mixed clones

Anti -Phosphotyrosine, mixed clones

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.(3,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes, including survival, proliferation, differentiation, and transformation.(3,6,7) The use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization, and purification of proteins that are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).(1,2,4,5)
Catalog #P4075-35
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blotting: 1:500 to 1:1,000
Immunoprecipitation:
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3G244
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in phosphate buffered saline, pH 7.4, 40% glycerol and 0.1% proclin.
PurityPurified by immunoaffinity chromatography.
ImmunogenTyrosine-phosphorylated proteins
SpecificityPY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective.
Species crossreactivityIt is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosinephosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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