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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP

Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.
(5,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes including survival, proliferation, differentiation, and transformation.(4,5,7) Moreover, the use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization and purification of proteins which are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).
(1,2,3,6)
Catalog #	P4075-36
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1ug/ml Pre-incubation of the antibody with 20 mM phosphotyrosine is recommended. Pre-incubation with 20mM phosphoserine or 20 mM phosphothreonine is ineffective.
	Immunoprecipitation: 5ug
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k+IgG,2b
Clone No	3G245,3G247
Host	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Phosphotyrosine, mixed clones.
Specificity	Recognizes tyrosine-phosphorylated proteins native and denatured states. No crossreactivity to phosphoserine or phosphothreonine.
Species Crossreactivity	Mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.