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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IP

Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation, and differentiation(1, 2).
Catalog #	P4075-39
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:2000
	Optimal dilutions to be determined by the researcher.
Positive controls	Mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	LgG2b
Clone No	3G248
Host	Mouse
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.1% proclin, 50% glycerol. Conjugated to HRP.
Purity	Purified from mouse ascites
Immunogen	Phosphotyrosine coupled to KLH.
Specificity	Reacts specifically with tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine. This antibody does not recognize phosphoserine or phosphothreonine.
Species Crossreactivity	(with phosphorylated proteins) Independent of the protein’s species of orgin. Positive controls include: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.