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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine (HRP)

Anti -Phosphotyrosine (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation, and differentiation(1, 2).
Catalog #P4075-39
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Positive controlsMouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeLgG2b
Clone No3G248
HostMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA and 0.1% proclin, 50% glycerol. Conjugated to HRP.
PurityPurified from mouse ascites
ImmunogenPhosphotyrosine coupled to KLH.
SpecificityReacts specifically with tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine. This antibody does not recognize phosphoserine or phosphothreonine.
Species Crossreactivity(with phosphorylated proteins) Independent of the proteinís species of orgin. Positive controls include: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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