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You are here:Home » Antibodies » Antibodies-Enzymes, Phospholipase » Anti -Phospholipase D (PLD)

Anti -Phospholipase D (PLD)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Activation of phospholipase D results in the generation of second messengers, phosphatidic acid and diglycerides, and appears to be involved in secretion, vesicle trafficking, mitosis and meiosis. In leukocytes, phospholipase D regulates cytoskeletal-dependent antimicrobial responses such as phagocytosis and oxidant generation.
Catalog #P4075-55
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsImmunoprecipitation: 4ug immunoprecipitates PLD1 and 2 from 500ug of Sf9 lysate expressing PLD 1 or 2.
Western Blot: 0.5-2ug/ml detects PLD in RIPA lysates from HeLa cells. HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P4075-55. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No4i56
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenFusion-protein corresponding to residues 326-504 of human PLD1
SpecificityRecognizes human PLD1, Mr 116kD and PLD2, Mr 100kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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