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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Cellular responses to a variety of extracellular signals occur through phosphorylation or dephosphorylation of intracellular proteins. Abnormal protein phosphorylation may be involved in the progression of numerous diseases, including many forms of cancer, immune system dysfunction and cardiovascular disease. Such changes in protein phosphorylation may be detected by immunocytochemical mapping of cells labeled with antiphosphoserine or antiphosphothreonine antibodies and can be correlated with cellular, electrophysiological or behavioral state changes.
Catalog #P4076
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot:1:500 detects serine phosphorylated proteins on 10ug of NGF PC12 lysates. NGF treated PC12 lysates were resolved by electrophoresis, transferred to PVDF membrane and probed wiht P4076. Proteins were visualized using a donkey anti-rabbbit secondary antibody (HRP) and a chemiluminescence detection system.
Immunoprecipitation (Tissue extracts): 10-20ug/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5ug/500ul of extracts.
ELISA (Kinase assay): 1:250-1:500
Immunohistochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlsNIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenPhosphoserine conjugated to KLH.
SpecificityRecognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not crossreact with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight crossreactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
Species CrossreactivityAll species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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