Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B Gel

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Catalog #	P4076-01
Applications	Suitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested.
Recommended Dilutions	Western Blot (Indirect): 1:500-1:1000
	Indirect ELISA: 1:4000
	Note: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
	Optimal dilutions to be determined by researcher.
Positive Control	Rat brain cortex and extract at ~50kD.
Hybridoma	Mouse myeloma cells with splenocytes from immunized mice.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3C171
Host	Mouse
Concentration	Not Determined
Form	Supplied as a liquid, 15mM sodium azide.
Purity	Ascites.
Immunogen	Phosphoserine conjugated to KLH.
Specificity	Recognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.