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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B
Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.
Catalog #P4076-01
ApplicationsSuitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested.
Recommended DilutionsWestern Blot (Indirect): 1:500-1:1000
Indirect ELISA: 1:8000-16,000
Note: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.
Optimal dilutions to be determined by researcher.
Positive ControlRat brain cortex and extract at ~50kD.
HybridomaMouse myeloma cells with splenocytes from immunized mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3C171
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid, 15mM sodium azide.
PurityAscites
ImmunogenPhosphoserine conjugated to KLH.
SpecificityRecognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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