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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IP
Phosphorylation and dephosphorylation of intracellular proteins occurs in response to extracellular signals. In certain disease states such as cancer, abnormal protein phosphorylation can occur.
Catalog #P4076-10F
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:1000-1:2000
Western Blot: For the detection of phosphoproteins, serine phosphatase inhibitors like 10mM sodium fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, 0.01% sodium azide, 50% gelatin/glycerol.
PurityPurified
ImmunogenPhosphoserine (KLH)
SpecificityRecognizes PHOSPHOSERINe, peptidyl-phosphoserine, and serine-phosphorylated proteins. Does not crossreact with ATP, phosphotyrosine, peptidyl phosphothreonine OR serine. Slight cross reactivity with free phosphothreonine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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