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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IP IH

Protein phosphorylation is an important post-translational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Catalog #	P4076-10G
Applications	Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 2-4ug/ml. Detects phosphorylation signal using human MMRU cells treated with 0.1uM okadaic acid.
	ELISA: 0.5-4ug/ml
	Immunoprecipitation: 10ug/mg
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.1% sodium azide.
Purity	Serum
Immunogen	Phosphoserine conjugated to KLH, and phosvitin mixture.
Specificity	Recognizes proteins phosphorylated on serine residues. Does not crossreact with phosphotyrosine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.