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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Protein phosphorylation is an important post-translational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Catalog #P4076-10G
ApplicationsSuitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 2-4ug/ml. Detects phosphorylation signal using human MMRU cells treated with 0.1uM okadaic acid.
ELISA: 0.5-4ug/ml
Immunoprecipitation: 10ug/mg
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenPhosphoserine conjugated to KLH, and phosvitin mixture.
SpecificityRecognizes proteins phosphorylated on serine residues. Does not crossreact with phosphotyrosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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