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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Catalog #P4076-11
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution. Replace by BSA.
Immunoprecipitation: 1-10ug per 10e6 vanadate-treated A431 cells
ELISA: 0.05ug/ml
Optimal dilutions to be determined by the researcher.
Source Tissue culture.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM,k
Clone No3C205
HostMouse
Concentration0.025mg/ml
FormSupplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute with 1ml sterile dH2O for 15 minutes at RT.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenPhosphoserine conjugated to KLH
SpecificityRecognizes Phosphoserine epitopes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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