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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing
extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for
the assembly of protein complexes or may alter the 3-dimensional protein structure thus
modulating enzymatic activity or the ability to undergo protein-protein-interactions.
Modification of proteins on serine residues is mediated by serine/threonine kinases.
Catalog #	P4076-19
Applications
	Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilution	Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells
	Western Blot: 1ug/ml with HRP/ECL detection. BSA/Tween 20 based blocking buffer. Do not use milk/casein for blocking and dilution!
	Optimal dilution to be determined by the researcher.
Positive Control Included	Yes
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	6D661
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS containing 0.09% sodium azide, PEG, and sucrose.
Purity	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Immunogen	Phosphoserine containing peptides.
Specificity	Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine.
Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species Crossreactivity	Human, mouse, rat and dog
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.