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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
Catalog #	P4076-22
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 0.05ug/ml
	Immunoprecipitation: 1-10ug/10e6 vanadate treated A431 cells
	Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer BPPT: 3%BSA, 1% PEG 4000, 1% PVP, 0.1% Tween 20, 2X PBS.
	Note: Do not use Milk/Casein for Blocking and dilution! Replace by BSA
	Optimal dilutions to be determined by the researcher.
Positive Control Included	P4076-22A: Phosphoserine Positive Control
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D664
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose.
Purity	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Immunogen	Phosphoserine containing peptides.
Specificity	Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine.
Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species Crossreactivity	Human, mouse, rat and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.