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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphoserine

Anti -Phosphoserine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
Catalog #P4076-22
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.05ug/ml
Immunoprecipitation: 1-10ug/10e6 vanadate treated A431 cells
Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer BPPT: 3%BSA, 1% PEG 4000, 1% PVP, 0.1% Tween 20, 2X PBS.
Note: Do not use Milk/Casein for Blocking and dilution! Replace by BSA
Optimal dilutions to be determined by the researcher.
Positive Control IncludedP4076-22A: Phosphoserine Positive Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D664
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, PEG, sucrose.
PurityPurified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
ImmunogenPhosphoserine containing peptides.
SpecificityRecognizes a broad range of serine-phosphorylated proteins in crude cell extracts. The antibody has a preference for positively charged amino acids directly next to the phosphoserine.
Please note that phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Species CrossreactivityHuman, mouse, rat and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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