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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the ã-P of ATP to a recipient protein that acts as a substrate.
This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Catalog #	P4076-50B
Positive Control	Mouse brain extract for Western Blotting. Synthetic phosphopeptide or phosvitin for ELISA.
Applications	Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.2.
Purity	Purified by immunoaffinity chromatography.
Immunogen	BSA and KLH-phosphoserine conjugates.
Specificity	Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not crossreact with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight crossreactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.