| Protein phosphorylation provides a signalling system that can be thought of as a kind of protein on/off switch for many cellular signalling pathways. Phosphorylation is observed on serine, threonine, tyrosine and histidine residues. Cellular networks underlying phosphorylation can be very complex and often occurs on multiple distinct sites on a given protein. |
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| Catalog # | P4076-50D |
| Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-2ug/ml for 2 hours at RT. |
| Immunoprecipitation: 2ug/mg protein lysate. |
| Immunohistochemistry (Paraffin): 1:20 for 10 minutes at RT. Boil tissue sections in 10mM citrate, pH 6 for 10 minutes folled by cooling at RT for 20 minutes. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Human breast carcinoma whole cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 9A354 |
| Host | Mouse |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide. |
| Purity | Purified |
| Immunogen | Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers. Cellular Localization: Cell Membrane and Cytoplasmic |
| Specificity | Recognizes serine, threonine, and tyrosine phosphorylated proteins. Species Crossreactivity: All. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
