You are here:Home
»
Antibodies
»
Abs to Amino Acids
»
Anti -Phosphoserine, Phosphothreonine, Phosphotyrosine (Ser, Thr, Tyr)
Anti -Phosphoserine, Phosphothreonine, Phosphotyrosine (Ser, Thr, Tyr)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| This antibody recognizes serine, threonine, and tyrosine phosphorylated proteins as determined by ELISA using phosphorylated peptides. Reactivity has also been shown with phosphorylated proteins in western blots. | | | Catalog # | P4076-61B | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-2ug/ml for 2hrs at RT | | Immunoprecipitation: 2ug/mg protein lysate | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:20 for 30min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | SPM101 | | Host | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Phosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers. | | Specificity | Recognizes serine, threonine, and tyrosine phosphorylated proteins in all species. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
