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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -Phosphoserine, Phosphothreonine, Phosphotyrosine (Ser, Thr, Tyr)

Anti -Phosphoserine, Phosphothreonine, Phosphotyrosine (Ser, Thr, Tyr)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Catalog #P4076-61B
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml for 2 hourrs at RT
Immunoprecipitation: 2ug/mg protein lysate
Immunohistochemistry (FFPE): 1:20 for 10 minutes at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlBreast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoSPM101
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPhosphoserine, phosphothreonine, and phosphotyrosine conjugated to protein carriers. Staining Pattern: Cell surface, cytoplasmic
SpecificityRecognizes serine, threonine, and tyrosine phosphorylated proteins in all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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