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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders.
Catalog #	P4077
Applications	Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Western Blot: 2ug/ml; Mouse brain lysate. Incubate at RT for 4 hrs.
	ELISA: 0.5ug/ml (kinase assay). Binding of Ly-pT-Gly-HRP
	(0.2ug/100ul/well) to an anti-phosphothreonine coated microplate (0.5ug/100ul/well) is inhibited by 95% with the addition of 2-10ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1mg/ml.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7, 0.01% sodium azide, 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Phosphothreonine conjugated to KLH and phosvitin.
Specificity	Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species crossreactivity: species independent
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.