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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
Catalog #	P4077-11
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:1000 to 1:5000, assayed against phosphothreonine conjugated BSA using IgG, H&L (HRP) Pab Gt xMo for detection.
	Western Blot: 1:500
	Optimal dilutions to be determined by the researcher.
Recommended secondary antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
	I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5H19
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid, 0.01% sodium azide before the addition of glycerol to 40%. No buffer or stabilizers added. No stabilizing proteins added.
Purity	Ascites
Immunogen	Phosphothreonine conjugated to KLH
Specificity	Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal crossreactivity is observed against phosphoserine or phosphotyrosine lysates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.