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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphothreonine (HRP)

Anti -Phosphothreonine (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B
Phosphorylated threonine (phosphothreonine) is an alpha amino acid that together with phosphoserine is one of the most ubiquitously modified amino acids in proteins. Many hormones, such as adrenaline (epinephrine), alter the activities of enzymes by stimulating the phosphorylation of serine and threonine, and these can have profound effects on cellular signal transduction, apoptosis and carcinogenesis.
Catalog #P4077-12F2
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 50% glycerol. Labeled with horseradish peroxidase (HRP).
PurityPurified
ImmunogenPhosphothreonine (KLH).
SpecificityRecognizes phosphothreonine in multiple forms including free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and in proteins of both unstimulated and stimulated cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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