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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphothreonine

Anti -Phosphothreonine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.
Catalog #P4077-14
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA: 0.05ug/ml
Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween20 based buffer.
Western Blot (BPPT): Recommended blocking buffer: 3% BSA, 1% PEG 4000, 1% PVP, 0.1% Tween20, 2x PBS. Do not use milk of casein for blocking.
Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells.
Optimal dilutions to be determined by the researcher.
Positive Control Cell Lysate (Included)P4076-22A: Phosphoserine Positive Control. Use 20ul/lane.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No6D665
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, PEG and sucrose.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenSynthetic Phosphothreonine containing peptides conjugated to KLH.
SpecificityRecognizes Phosphothreonine. Species Crossreactivity: human, mouse, rat, canine. Recognizes a broad range of threonine-phosphorylated proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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