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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IP

Catalog #	P4077-15
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	ELISA: 0.05ug/ml
	Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: BSA, Tween 20based blocking buffer.
	Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells.
	Optimal dilutions to be determined by the researcher.
Positive Control Cell Lysate (Included)	P4076-22A: Phosphoserine Positive Control. Use 20ul/lane
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D666
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide, PEG, sucrose.
Purity	Purified by thiophilic adsorption and size exclusion chromatography.
Immunogen	Phosphothreonine containing peptides (KLH).
Specificity	Recognizes Phosphothreonine. Species Crossreactivity: human, mouse, rat, canine. Recognizes a broad range of threonine-phosphorylated proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.