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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -Phosphothreonine

Anti -Phosphothreonine


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Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Catalog #P4077-15
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA: 0.05ug/ml
Western Blot: 1ug/ml for HRPO/ECL detection. Recommended blocking buffer: BSA, Tween 20 based blocking buffer.
Immunoprecipitation: 1-10ug per 10e6 pervanadate-treated A431 cells.
Optimal dilutions to be determined by the researcher.
Positive Control Cell Lysate (Included)P4076-22A: Phosphoserine Positive Control. Add 100ul SDS-PAGE sample buffer and incubate at 90°C for 5 minutes. Use 20ul/lane
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No6D666
FormSupplied as a liquid in PBS, 0.09% sodium azide, PEG, sucrose.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenPhosphothreonine containing peptides. Conjugated to KLH.
SpecificityRecognizes Phosphothreonine. Species Crossreactivity: human, mouse, rat, canine. Recognizes a broad range of threonine-phosphorylated proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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