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You are here:Home » Molecular Biology » MB-Amino Acids » Phosphothreonine (O-Phospho-L-Threonine, Anti-Phosphothreonine Inhibitor)

Phosphothreonine (O-Phospho-L-Threonine, Anti-Phosphothreonine Inhibitor)

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Specifications

This anti-phosphothreonine inhibitor can be utilized in ELISA and Western Blot applications to block the specific interaction of antiphosphothreonine primary antibodies with threonine phosphorylated proteins(1,2).
Catalog #P4077-50
ApplicationsSuitable for use in ELISA and Western Blot Other applications not tested.
Recommended UsageThis inhibitor is recommend for use with anti-phosphothreonine primary antibodies to distinguish specific versus non-specific binding of these antibodies in ELISA and Western blotting experiments. The inhibitor should be used at a final concentration of 20 mM to block the specific binding of an anti-phosphothreonine primary antibody. For Western blotting, inhibitor solution and primary antibody are added to a BSA containing blocking solution and pre-incubated for 5- 10 minutes prior to application to the membrane. Incubations are typically carried out for 1 hr at room temperature. Following the incubation with the inhibitor/antibody solution, standard procedures are followed for blot washing and incubation with a secondary antibody.
Storage and StabilityStore at 4°C or colder. Stable for at least 6 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
FormSupplied as a liquid (40mM O-Phospho-L-Threonine in PBS, pH 7.4, containing 0.1% sodium azide).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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