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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.
Catalog #	P4078-01E
	The binding of P4078-01E to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.
	The affinity of P4078-01E for phosphotyrosine is 10e-6 to 10e-7 M.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:1,000-1:4,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
	Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	10F282
Host	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.4, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Phosphotyrosine.
Specificity	Recognizes phosphotyrosine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.