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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Tyrosine residues are phosphorylated by a number of oncogene protein kinases and growth factor receptors. P4078-02 may be used to study the biological significance of such protein modifications.
Catalog #	P4078-02
Applications	Suitable for use in Western Blot, isolation of phosphotyrosine containing proteins and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:100
	High concentrations of salt (0.2M) and divalent cations (1mM) may inhibit binding of this antibody.
	Optimal dilutions to be determined by the researcher.
Note	Fluorescein, which has a maximum absorbance at 492nm and an emission maximum at 518nm has been covalently attached to anti-Phosphotyrosine and purified to assure optimal fluorochrome/protein (F/P) molar ratios.
Source	Tissue culture
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	1.BB.49
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with FITC.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Phosphotyrosine
Specificity	Recognizes phosphotyrosine containing proteins with high affinity and specificity. Does not react with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.