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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases.
Catalog #P4078-05E
ApplicationsSuitable for use in ELISA, Immunoprecipitation, and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.1ug/ml for HRP/ECL detection
Immunoprecipitation: 1-10ug per 10e6 vanadate-treated A431 cells
Optimal dilutions to be determined by the researcher.
Positive Control (supplied)Phosphotyrosine Molecular Weight Marker. Supplied as a lyophilized powder. Reconstitute with 200ul sterile, ddH2O. After complete solubilization of the proteins, add 200ul SDS-PAGE sample buffer and incubate at 90°C for 5 minutes. For WB applications, use 20ul/lane (mini gel) for HRP/DAB detection and 10ul/lane (mini gel) for HRP/ECL detection. Do not use NC membranes.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No9L544
HostMouse
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a powder lyophilized from PBS containing PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml ddH2O.
PurityPurified by thiophilic adsorption and size exclusion chromatography
ImmunogenPhosphotyrosine
SpecificityRecognizes a broad spectrum of tyrosine-phosphorylated proteins in crude cell extracts and may be particularly well suited for the detection/screening of tyrosine phosphorylated proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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