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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IF

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a
phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple
sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected
through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Catalog #	P4078-05F
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10F757
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography
Immunogen	Phosphotyrosine, alanine and glyceine in a 1
dimentrylaminopropyl) carbodiimide.
Specificity	Recognizes Phosphotyrosine. Does not cross react with phosphoserine or phosphothreonine.
Species Crossreactivity	Not species specific.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.