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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -Phosphotyrosine (Biotin)

Anti -Phosphotyrosine (Biotin)


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Clone Host Grade Applications
Monoclonal Mouse Purified B IP IC
Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #P4078-08
ApplicationsSuitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
Recommended DilutionWestern Blotting: 0.52ug/ml will detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Immunoprecipitation: 4ug, used in conjunction with Streptavidin-Agarose, will immunoprecipitate phosphotyrosine containing proteins from a lysate of EGF-stimulated A431 cells; to preserve phosphotyrosine, add 0.2mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry: 510ug/ml will give a positive immunostaining of EGF-stimulated A431 cells that have been fixed with ethanol:acetic acid
Clone TypeMonoclonal
Clone No0.T.162
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride containing 0.05% sodium azide. Conjugated to Biotin.
PurityPurified IgG
SpecificityReacts with Phosphotyrosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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