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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IP IC

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #	P4078-08
Applications	Suitable for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
Western Blotting	0.5–2ug/ml will detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Immunoprecipitation	4ug, used in conjunction with Streptavidin-Agarose, will immunoprecipitate phosphotyrosine containing proteins from a lysate of EGF-stimulated A431 cells; to preserve phosphotyrosine, add 0.2mM sodium orthovanadate to the lysis buffer.
Immunocytochemistry	5–10ug/ml will give a positive immunostaining of EGF-stimulated A431 cells that have been fixed with ethanol:acetic acid
CAS Number	n/a
Clone Type	Monoclonal
Clone No	0.T.162
Host	Mouse
Form	Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride containing 0.05% sodium azide. Conjugated to Biotin.
Purity	Purified IgG
Immunogen	Phosphotyrosine
Specificity	Reacts with Phosphotyrosine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.