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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine (FITC)

Anti -Phosphotyrosine (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IC
Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #P4078-09
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 5ug/ml. Detects phosphotyrosine containing proteins in EGF-stimulated human A431 cells.
Optimal dilutions to be determined by researcher.
HybridomaFOXY-NY (a NS-1 derivative) crossed with spleen cells from immunized mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No0.T.162
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
PurityAffinity Purified by Protein G affinity chromatography.
ImmunogenPhosphotyramine (KLH coupled)
SpecificityRecognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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