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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IC

Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #	P4078-09
Applications	Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunocytochemistry: 5ug/ml. Detects phosphotyrosine containing proteins in EGF-stimulated human A431 cells.
	Optimal dilutions to be determined by researcher.
Hybridoma	FOXY-NY (a NS-1 derivative) crossed with spleen cells from immunized mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	0.T.162
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
Purity	Affinity Purified by Protein G affinity chromatography.
Immunogen	Phosphotyramine (KLH coupled)
Specificity	Recognizes phosphotyrosine and protein-phosphotyrosine. Does not react with phosphoserine or phosphothreonine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.