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Phosphotyrosine Control, EGF-Stimulated A431 Cell
Phosphotyrosine Control, EGF-Stimulated A431 Cell
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| Catalog # | P4078-10 | | Applications | Positive control for anti-phosphotyrosine antibodies. Suitable for use in Western Blot, Immunoprecipitation. | | Recommended Dilution | Western Blot: Detects the phosphorylated EGF receptor (170kD) and other tyrosine phosphorylated proteins in 20ug of the preparation. Add an equal volume of Laemmli reducing sample buffer to 5-25ul of lysate a;nd boil for 5 min to reduce the preparation. | | Immunoprecipitation Positive Control | This preparation may also be used for immunoprecipitation with anti-phosphotyrosine agarose. | | Storage and Stability | Store at -20ºC Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. | | Source | Human | | Purity | Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. | | Concentration | 2mg/ml | | Form | Supplied as a frozen solution in RIPA sample buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EGTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM Na3VO4, 1mM NaF). | | | | Intended for research use only, not for use in human, therapeutic or diagnostic applications. | | |
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