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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP IC
Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #P4078-12
ApplicationsSuitable for use in ELISA (kinase phophatase assay), Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.4-1ug/ml
Immunoprecipitation: 10ug/100ul
Immunocytochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No2Q267
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
PurityPurified
ImmunogenHapten O-phopshotyramine
SpecificitySpecifically recognizes (with high affinity) proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50:10e-6M, ELISA). Does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulfated (23-25) cholecystokinine) or Na-benzoate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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