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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Protein kinases that phosphorylate the hydroxyl group of tyrosine residues of proteins have been implicated in cell transformation by some retroviruses and in regulation of normal cell growth by some polypeptide growth factors (1). Antibodies to tyrosine phosphorylated proteins are helpful in facilitating the identification of tyrosine kinase substrates (2).
Catalog #P4078-26
ApplicationsSuitable for use in Western Blotting. Other applications not tested.
Recommended DilutionWestern Blotting: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
Concentration0.25mg/ml
FormSupplied as a liquid in TBS, 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPhosphotyrosine moieties conjugated to KLH.
SpecificityRecognizes proteins and peptides that contain phosphorylated tyrosine residues. Does not recognize phosphoserine, phosphothreonine or non-phosphorylated tyrosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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