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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine

Anti -Phosphotyrosine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IP IC
Protein tyrosine phosphorylation has been the subject of intense investigation for over two decades. Tyrosine phosphorylation is catalyzed by members of the tyrosine kinase superfamily. To date, approximately 90 members of this superfamily have been identified in the human genome. Levels of phosphorylation of tyrosine residues within cellular proteins are observed to increase in response to growth factors, cytokines, insulin, extracellular matrix components, and other stimuli. Tyrosine phosphorylation has several effects, including the regulation of enzyme activity, as well as the localization of enzyme activity through the generation of docking sites for proteins bearing SH2 domains.
Catalog #P4078-38
ApplicationsSuitable for use in ELISA, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA: 0.05ug/ml
Western Blot (AP/BCIP/NBT (MTT)): 1ug/ml
Western Blot (HRPO/ECL): 0.1ug/ml
Immunoprecipitation: 1-10ug per 10e6 vanadate treated A431 cells
Immunocytochemistry: 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Western Blot Positive Control IncludedP4078-38A: Phosphotyrosine, Molecular Weight Marker
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6D670
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide, PEG and sucrose.
PurityPurified by size exclusion chromatography.
ImmunogenPhosphotyrosine conjugated to KLH
SpecificityRecognizes phosphotyrosine in the context of the surrounding amino acids, showing high preference for phosphorylated EGF receptor. Species crossreactivity: human, mouse, rat, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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