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P4079-03D Goat Anti-Phox2b (Paired Mesoderm Homeobox Protein 2B, Neuroblastoma Phox, NBPhox, PHOX2B Homeodomain Protein, Paired-like Homeobox 2B, PMX2B)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
Q99453
Isotype
IgG
Grade
Affinity Purified
Applications
IC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

PHOX2B is a 33-35kD member of the paired homeobox family of transcription factors. It is not actually a mesoderm-associated protein, but is instead apparently restricted to neuronal precursors and mature neurons. It is found in glutamatergic neurons of the NTS and in neurons that demarcate the respiratory chemoreception pathway. In addition, PHOX2B is essential to the development of noradrenergic adrenal chromaffin and sympathetic motor neurons. Human PHOX2B is 314aa in length. It contains one DNA binding homeobox domain aa98-157 and two poly-Ala sequences, one between aa159-167 and another between aa241-260. Alanine extensions involving anywhere from 5 to 11 Ala residues may exist. These change the nature of the PHOX2B molecule. While wild-type PHOX2B is nuclear, Ala extensions promote its retention in the cytoplasm with loss of activity. PHOX2B may potentially form oligomers with itself, or it paralogue, PHOX2A. Human and mouse PHOX2B are identical in aa sequence

Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-derived, recombinant protein corresponding to Met1-Glu94 of human PHOX2B.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PHOX2B.
Conjugates
Pricing
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