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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IF

Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. Also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of this clone for phosphotyrosine is approximately 10e-6 to 10e-7 M. Binding of this Ab to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3
Catalog #	P4079D
Applications	Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:1000-1:4000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b,k
Clone No	5K212
Host	Mouse
Concentration	Not determined.
Form	Supplied as a liquid in 50% PBS, pH 7.4, 50% glycerol. Labeled with HRP. No preservative added.
Purity	Purified
Immunogen	Phosphotyrosine (HRP).
Specificity	Recognizes phosphorylated tyrosine and phenylphosphate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.