Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Amino Acids » Anti -Phosphotyrosine (PE)

Anti -Phosphotyrosine (PE)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IF
Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. Also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of this clone for phosphotyrosine is approximately 10e-6 to 10e-7 M. Binding of this Ab to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.1-3
Catalog #P4079F
Applications Detection of phosporylated tyrosine-containing proteins on Western blots 1
Immunoaffinity purification of tyrosine-phosporylated proteins 1
Quantitation of phosphotyrosine by Enzyme-Linked-Immunosorbent-Assay (ELISA)1
Immunofluorescence analysis 4
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No5K212
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
PurityPurified
SpecificityRecognizes phosphorylated tyrosine and phenylphosphate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links