Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Transcription Factors, STAT » Anti -PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-AL

Anti -PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting
protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682,
miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-AL

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx- ) is a 45-70kD member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK-1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa11-45), a zinc-finger region (aa331-408), a SUMO-binding motif (aa467-472) and an NLS (aa484-492). There are multiple splice variants. PIAS2x- /ARIP3 shows a 22 aa substitution for aa 551-621, while PIAS-NY shows a 12 aa substitution for aa 1-8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504-621, and an alternate start site at Met10. Over aa 1-550, human PIAS2 shares 98% aa identity with mouse PIAS2.
Catalog #P4085-11
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, E. coli-derived recombinant human PIAS2 aa 1-550; Accession# O75928.
SpecificityDetects endogenous human PIAS2 in Western blot with an approximate molecular weight of 63-70kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links