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You are here:Home » Antibodies » Antibodies-Transcription Factors, STAT » Anti -PIAS1, CT E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) (Azide free) (HRP)

Anti -PIAS1, CT E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein
1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1,
DDXBP1) (Azide free) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
Catalog #P4086-01A-HRP
ApplicationsSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note Applications are based on unconjugated antibody.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Labeled with HRP.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenKLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 100-130 in the N-terminal region of human PIAS1, UniProt Accession #O75925.
SpecificityRecognizes human PIAS1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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