Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL,
dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif
Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP |
|
| GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the Golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastoma. The resulting GOPC-ROS fusion protein is targeted to the Golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8). | | | Catalog # | P4207-51E | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to residues surrounding Met266 of human GOPC protein (KLH-coupled). | | Specificity | Recongnizes endogenous levels of human GOPC following IP enrichment. Species crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL, dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT |
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